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mouse bone marrow mesenchymal stem cells (bmsc)  (Procell Inc)

 
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    Procell Inc mouse bone marrow mesenchymal stem cells (bmsc)
    Mouse Bone Marrow Mesenchymal Stem Cells (Bmsc), supplied by Procell Inc, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/mouse+bone+marrow+mesenchymal+stem+cells+(bmsc)/mouse+bone+marrow+mscs/pm40543383-82-0-17
    Average 90 stars, based on 1 article reviews
    mouse bone marrow mesenchymal stem cells (bmsc) - by Bioz Stars, 2026-09
    90/100 stars

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    Article Title: Multifunctional bioactive metal phenolic networks (MPN) with copper ion retardation and dual-peptides for enhanced osteointegration.
    Article Snippet: Titanium rods are widely used as orthopedic implants, but their biologically inert surface limits their effectiveness in bone healing.. To enhance osseointegration, implants need to be multifunctional, capable of modulating cellular behaviors such as early adhesion, migration, angiogenesis, and bone formation.. In this study, copper ion-doped phenolic networks (P/Cu MPNs) were developed as a platform for grafting bioactive peptides (OGP and RGD), terminally coupled with K6 (hexameric lysine), to create a multifunctional bioactive coating (RGD/OGP@P/Cu) aimed at improving osteointegration.

    Article Title: In situ biomineralization reinforcing anisotropic nanocellulose scaffolds for guiding the differentiation of bone marrow-derived mesenchymal stem cells.
    Article Snippet: Nanocellulose (NC) is a promising biopolymer for various biomedical applications owing to its biocompatibility and low toxicity.. However, it faces challenges in tissue engineering (TE) applications due to the inconsistency of the microenvironment within the NC-based scaffolds with target tissues, including anisotropy microstructure and biomechanics.. To address this challenge, a facile swelling-induced nanofiber alignment and a novel in situ biomineralization reinforcement strategies were developed for the preparation of NC-based scaffolds with tunable anisotropic structure and mechanical strength for guiding the differentiation of bone marrow-derived mesenchymal stem cells for potential TE application.

    Derivative Assay:

    Article Title: Engineered migrasomes provide a robust and thermally stable vaccination platform
    Article Snippet: Reagent type (species) or resource Designation Source or reference Identifiers Additional information Cell line (Rattus norvegicus) NRK ATCC CRL- 6509 Cell line (Homo sapiens) HEK 293T ATCC CRL- 3216 Wang, Wang et al. eLife 2024;13:RP97621. .. DOI: https://doi.org/10.7554/eLife.97621 14 of 21 Reagent type (species) or resource Designation Source or reference Identifiers Additional information Cell line (Mus musculus) MCA- 205 STR profiling, authenticated by Procell Life Science & Technology Cell line (Cercopithecus aethiops) Vero ATCC CCL- 81 Cell line (H. sapiens) ADSC Procell CP- H202 Human Adipose- derived Mesenchymal Stem Cells Cell line (H. sapiens) BMSC Procell CP- H166 Human Bone Marrow Mesenchymal Stem Cells Antibody Anti- ovalbumin (mouse monoclonal) Santa Cruz sc- 80589 ELISA (1:4000– 1:512,000), WB (1:1000) Antibody Anti- Spike (mouse monoclonal) Sino Biological 40591- MM42 ELISA (1:5000– 1:640,000), WB (1:1000) Antibody Anti- Spike (chimeric MAb) Sino Biological 40150- D001 IF (1:100) Recombinant DNA reagent pB- mOVA- mCherry (plasmid) This paper pB- mCherry derived plasmid Recombinant DNA reagent pB- S- mCherry (plasmid) This paper pB- mCherry derived plasmid Recombinant DNA reagent pB- Tspan4- GFP (plasmid) Jiao et al., 2021 pB- GFP derived plasmid Peptide, recombinant protein Ovalbumin Sigma A5503 Chemical compound, drug Latrunculin A Cayman 10010630 0.25–2 μM Other Imject Alum Thermo Fisher 77161 Other Fibronectin Sigma F0895 10 μg/ml for imaging; 4 μg/ml for eMigrasome preparation Other 40 kDa Dextran- TMR Thermo Fisher D1842 (25 μg/ml) Continued .. For the construction of pB- Tspan4- GFP, pB- PD1- mCherry, pB- S- mCherry, pmCherry- N1- Tspan4, pmCherry- N1- CD82, and pmCherry- N1- Tspan1, the coding sequence of the corresponding genes was amplified by PCR and subsequently inserted into the vector using a One Step Cloning Kit (Vazyme, C112).

    Enzyme-linked Immunosorbent Assay:

    Article Title: Engineered migrasomes provide a robust and thermally stable vaccination platform
    Article Snippet: Reagent type (species) or resource Designation Source or reference Identifiers Additional information Cell line (Rattus norvegicus) NRK ATCC CRL- 6509 Cell line (Homo sapiens) HEK 293T ATCC CRL- 3216 Wang, Wang et al. eLife 2024;13:RP97621. .. DOI: https://doi.org/10.7554/eLife.97621 14 of 21 Reagent type (species) or resource Designation Source or reference Identifiers Additional information Cell line (Mus musculus) MCA- 205 STR profiling, authenticated by Procell Life Science & Technology Cell line (Cercopithecus aethiops) Vero ATCC CCL- 81 Cell line (H. sapiens) ADSC Procell CP- H202 Human Adipose- derived Mesenchymal Stem Cells Cell line (H. sapiens) BMSC Procell CP- H166 Human Bone Marrow Mesenchymal Stem Cells Antibody Anti- ovalbumin (mouse monoclonal) Santa Cruz sc- 80589 ELISA (1:4000– 1:512,000), WB (1:1000) Antibody Anti- Spike (mouse monoclonal) Sino Biological 40591- MM42 ELISA (1:5000– 1:640,000), WB (1:1000) Antibody Anti- Spike (chimeric MAb) Sino Biological 40150- D001 IF (1:100) Recombinant DNA reagent pB- mOVA- mCherry (plasmid) This paper pB- mCherry derived plasmid Recombinant DNA reagent pB- S- mCherry (plasmid) This paper pB- mCherry derived plasmid Recombinant DNA reagent pB- Tspan4- GFP (plasmid) Jiao et al., 2021 pB- GFP derived plasmid Peptide, recombinant protein Ovalbumin Sigma A5503 Chemical compound, drug Latrunculin A Cayman 10010630 0.25–2 μM Other Imject Alum Thermo Fisher 77161 Other Fibronectin Sigma F0895 10 μg/ml for imaging; 4 μg/ml for eMigrasome preparation Other 40 kDa Dextran- TMR Thermo Fisher D1842 (25 μg/ml) Continued .. For the construction of pB- Tspan4- GFP, pB- PD1- mCherry, pB- S- mCherry, pmCherry- N1- Tspan4, pmCherry- N1- CD82, and pmCherry- N1- Tspan1, the coding sequence of the corresponding genes was amplified by PCR and subsequently inserted into the vector using a One Step Cloning Kit (Vazyme, C112).

    Western Blot:

    Article Title: Engineered migrasomes provide a robust and thermally stable vaccination platform
    Article Snippet: Reagent type (species) or resource Designation Source or reference Identifiers Additional information Cell line (Rattus norvegicus) NRK ATCC CRL- 6509 Cell line (Homo sapiens) HEK 293T ATCC CRL- 3216 Wang, Wang et al. eLife 2024;13:RP97621. .. DOI: https://doi.org/10.7554/eLife.97621 14 of 21 Reagent type (species) or resource Designation Source or reference Identifiers Additional information Cell line (Mus musculus) MCA- 205 STR profiling, authenticated by Procell Life Science & Technology Cell line (Cercopithecus aethiops) Vero ATCC CCL- 81 Cell line (H. sapiens) ADSC Procell CP- H202 Human Adipose- derived Mesenchymal Stem Cells Cell line (H. sapiens) BMSC Procell CP- H166 Human Bone Marrow Mesenchymal Stem Cells Antibody Anti- ovalbumin (mouse monoclonal) Santa Cruz sc- 80589 ELISA (1:4000– 1:512,000), WB (1:1000) Antibody Anti- Spike (mouse monoclonal) Sino Biological 40591- MM42 ELISA (1:5000– 1:640,000), WB (1:1000) Antibody Anti- Spike (chimeric MAb) Sino Biological 40150- D001 IF (1:100) Recombinant DNA reagent pB- mOVA- mCherry (plasmid) This paper pB- mCherry derived plasmid Recombinant DNA reagent pB- S- mCherry (plasmid) This paper pB- mCherry derived plasmid Recombinant DNA reagent pB- Tspan4- GFP (plasmid) Jiao et al., 2021 pB- GFP derived plasmid Peptide, recombinant protein Ovalbumin Sigma A5503 Chemical compound, drug Latrunculin A Cayman 10010630 0.25–2 μM Other Imject Alum Thermo Fisher 77161 Other Fibronectin Sigma F0895 10 μg/ml for imaging; 4 μg/ml for eMigrasome preparation Other 40 kDa Dextran- TMR Thermo Fisher D1842 (25 μg/ml) Continued .. For the construction of pB- Tspan4- GFP, pB- PD1- mCherry, pB- S- mCherry, pmCherry- N1- Tspan4, pmCherry- N1- CD82, and pmCherry- N1- Tspan1, the coding sequence of the corresponding genes was amplified by PCR and subsequently inserted into the vector using a One Step Cloning Kit (Vazyme, C112).

    Recombinant:

    Article Title: Engineered migrasomes provide a robust and thermally stable vaccination platform
    Article Snippet: Reagent type (species) or resource Designation Source or reference Identifiers Additional information Cell line (Rattus norvegicus) NRK ATCC CRL- 6509 Cell line (Homo sapiens) HEK 293T ATCC CRL- 3216 Wang, Wang et al. eLife 2024;13:RP97621. .. DOI: https://doi.org/10.7554/eLife.97621 14 of 21 Reagent type (species) or resource Designation Source or reference Identifiers Additional information Cell line (Mus musculus) MCA- 205 STR profiling, authenticated by Procell Life Science & Technology Cell line (Cercopithecus aethiops) Vero ATCC CCL- 81 Cell line (H. sapiens) ADSC Procell CP- H202 Human Adipose- derived Mesenchymal Stem Cells Cell line (H. sapiens) BMSC Procell CP- H166 Human Bone Marrow Mesenchymal Stem Cells Antibody Anti- ovalbumin (mouse monoclonal) Santa Cruz sc- 80589 ELISA (1:4000– 1:512,000), WB (1:1000) Antibody Anti- Spike (mouse monoclonal) Sino Biological 40591- MM42 ELISA (1:5000– 1:640,000), WB (1:1000) Antibody Anti- Spike (chimeric MAb) Sino Biological 40150- D001 IF (1:100) Recombinant DNA reagent pB- mOVA- mCherry (plasmid) This paper pB- mCherry derived plasmid Recombinant DNA reagent pB- S- mCherry (plasmid) This paper pB- mCherry derived plasmid Recombinant DNA reagent pB- Tspan4- GFP (plasmid) Jiao et al., 2021 pB- GFP derived plasmid Peptide, recombinant protein Ovalbumin Sigma A5503 Chemical compound, drug Latrunculin A Cayman 10010630 0.25–2 μM Other Imject Alum Thermo Fisher 77161 Other Fibronectin Sigma F0895 10 μg/ml for imaging; 4 μg/ml for eMigrasome preparation Other 40 kDa Dextran- TMR Thermo Fisher D1842 (25 μg/ml) Continued .. For the construction of pB- Tspan4- GFP, pB- PD1- mCherry, pB- S- mCherry, pmCherry- N1- Tspan4, pmCherry- N1- CD82, and pmCherry- N1- Tspan1, the coding sequence of the corresponding genes was amplified by PCR and subsequently inserted into the vector using a One Step Cloning Kit (Vazyme, C112).

    Plasmid Preparation:

    Article Title: Engineered migrasomes provide a robust and thermally stable vaccination platform
    Article Snippet: Reagent type (species) or resource Designation Source or reference Identifiers Additional information Cell line (Rattus norvegicus) NRK ATCC CRL- 6509 Cell line (Homo sapiens) HEK 293T ATCC CRL- 3216 Wang, Wang et al. eLife 2024;13:RP97621. .. DOI: https://doi.org/10.7554/eLife.97621 14 of 21 Reagent type (species) or resource Designation Source or reference Identifiers Additional information Cell line (Mus musculus) MCA- 205 STR profiling, authenticated by Procell Life Science & Technology Cell line (Cercopithecus aethiops) Vero ATCC CCL- 81 Cell line (H. sapiens) ADSC Procell CP- H202 Human Adipose- derived Mesenchymal Stem Cells Cell line (H. sapiens) BMSC Procell CP- H166 Human Bone Marrow Mesenchymal Stem Cells Antibody Anti- ovalbumin (mouse monoclonal) Santa Cruz sc- 80589 ELISA (1:4000– 1:512,000), WB (1:1000) Antibody Anti- Spike (mouse monoclonal) Sino Biological 40591- MM42 ELISA (1:5000– 1:640,000), WB (1:1000) Antibody Anti- Spike (chimeric MAb) Sino Biological 40150- D001 IF (1:100) Recombinant DNA reagent pB- mOVA- mCherry (plasmid) This paper pB- mCherry derived plasmid Recombinant DNA reagent pB- S- mCherry (plasmid) This paper pB- mCherry derived plasmid Recombinant DNA reagent pB- Tspan4- GFP (plasmid) Jiao et al., 2021 pB- GFP derived plasmid Peptide, recombinant protein Ovalbumin Sigma A5503 Chemical compound, drug Latrunculin A Cayman 10010630 0.25–2 μM Other Imject Alum Thermo Fisher 77161 Other Fibronectin Sigma F0895 10 μg/ml for imaging; 4 μg/ml for eMigrasome preparation Other 40 kDa Dextran- TMR Thermo Fisher D1842 (25 μg/ml) Continued .. For the construction of pB- Tspan4- GFP, pB- PD1- mCherry, pB- S- mCherry, pmCherry- N1- Tspan4, pmCherry- N1- CD82, and pmCherry- N1- Tspan1, the coding sequence of the corresponding genes was amplified by PCR and subsequently inserted into the vector using a One Step Cloning Kit (Vazyme, C112).

    Imaging:

    Article Title: Engineered migrasomes provide a robust and thermally stable vaccination platform
    Article Snippet: Reagent type (species) or resource Designation Source or reference Identifiers Additional information Cell line (Rattus norvegicus) NRK ATCC CRL- 6509 Cell line (Homo sapiens) HEK 293T ATCC CRL- 3216 Wang, Wang et al. eLife 2024;13:RP97621. .. DOI: https://doi.org/10.7554/eLife.97621 14 of 21 Reagent type (species) or resource Designation Source or reference Identifiers Additional information Cell line (Mus musculus) MCA- 205 STR profiling, authenticated by Procell Life Science & Technology Cell line (Cercopithecus aethiops) Vero ATCC CCL- 81 Cell line (H. sapiens) ADSC Procell CP- H202 Human Adipose- derived Mesenchymal Stem Cells Cell line (H. sapiens) BMSC Procell CP- H166 Human Bone Marrow Mesenchymal Stem Cells Antibody Anti- ovalbumin (mouse monoclonal) Santa Cruz sc- 80589 ELISA (1:4000– 1:512,000), WB (1:1000) Antibody Anti- Spike (mouse monoclonal) Sino Biological 40591- MM42 ELISA (1:5000– 1:640,000), WB (1:1000) Antibody Anti- Spike (chimeric MAb) Sino Biological 40150- D001 IF (1:100) Recombinant DNA reagent pB- mOVA- mCherry (plasmid) This paper pB- mCherry derived plasmid Recombinant DNA reagent pB- S- mCherry (plasmid) This paper pB- mCherry derived plasmid Recombinant DNA reagent pB- Tspan4- GFP (plasmid) Jiao et al., 2021 pB- GFP derived plasmid Peptide, recombinant protein Ovalbumin Sigma A5503 Chemical compound, drug Latrunculin A Cayman 10010630 0.25–2 μM Other Imject Alum Thermo Fisher 77161 Other Fibronectin Sigma F0895 10 μg/ml for imaging; 4 μg/ml for eMigrasome preparation Other 40 kDa Dextran- TMR Thermo Fisher D1842 (25 μg/ml) Continued .. For the construction of pB- Tspan4- GFP, pB- PD1- mCherry, pB- S- mCherry, pmCherry- N1- Tspan4, pmCherry- N1- CD82, and pmCherry- N1- Tspan1, the coding sequence of the corresponding genes was amplified by PCR and subsequently inserted into the vector using a One Step Cloning Kit (Vazyme, C112).



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    Identification of <t>BMSCs-exosomes.</t> (A) Size distribution of BMSCs-exosomes. (B) transmission electron microscopy image of BMSCs-exosomes. Scale bars: 100 nm. (C) Western blot results of exosome surface proteins
    Primary Mouse Bone Marrow Mesenchymal Stem Cells Bmscs, supplied by ATCC, used in various techniques. Bioz Stars score: 94/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    mouse bone marrow mesenchymal stem cells (bmscs)  (Cyagen Biosciences)
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    Cyagen Biosciences mouse bone marrow mesenchymal stem cells (bmscs)
    Identification of <t>BMSCs-exosomes.</t> (A) Size distribution of BMSCs-exosomes. (B) transmission electron microscopy image of BMSCs-exosomes. Scale bars: 100 nm. (C) Western blot results of exosome surface proteins
    Mouse Bone Marrow Mesenchymal Stem Cells (Bmscs), supplied by Cyagen Biosciences, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/mouse+bone+marrow+mesenchymal+stem+cells+(bmsc)/bmscs/pm39047413-91-0-10
    Average 90 stars, based on 1 article reviews
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    Image Search Results


    Asp 10 SAC4A enables hypoxia-activated senolysis and promotes osteogenic differentiation in vitro. A ) Heatmap of combination index (CI) values at different D: Q ratios and fraction affected (Fa) levels, showing synergistic effects of Dasatinib and Quercetin (DQ). B ) Cell viability curves of normal BMSCs, senescent BMSCs (Sn-BMSCs), and Sn-BMSCs under hypoxia treated with vehicle, free DQ, Asp 10 SAC4A, or DQ@Asp 10 SAC4A. C ) Representative SA-β-Gal staining images of senescent cells treated with different formulations under normoxic and hypoxic conditions, and quantification of SA-β-Gal-positive area (%) ( n = 4/group). Scale bar: 100 μm. D–F ) Representative Western blot images ( D ) and quantitative analyses ( E , F ) of senescence markers P16 and P21 expression in different treatment groups ( n = 3/group). G ) Representative alkaline phosphatase (ALP, upper panel) and Alizarin Red staining (ARS, lower panel) images demonstrating osteogenic differentiation after indicated treatments under normoxic and hypoxic conditions. H–J ) Representative Western blot images ( H ) and quantitative analyses ( I , J ) showing protein expression levels of osteogenic markers RUNX2 and osteopontin (OPN) ( n = 3/group). (Data are presented as mean ± SD; * P < 0.05, ** P < 0.01, *** P < 0.001; n = 3–4/group)

    Journal: Journal of Nanobiotechnology

    Article Title: Supramolecular delivery of senolytics enables targeted anti-senescence therapy and accelerated fracture healing

    doi: 10.1186/s12951-026-04138-2

    Figure Lengend Snippet: Asp 10 SAC4A enables hypoxia-activated senolysis and promotes osteogenic differentiation in vitro. A ) Heatmap of combination index (CI) values at different D: Q ratios and fraction affected (Fa) levels, showing synergistic effects of Dasatinib and Quercetin (DQ). B ) Cell viability curves of normal BMSCs, senescent BMSCs (Sn-BMSCs), and Sn-BMSCs under hypoxia treated with vehicle, free DQ, Asp 10 SAC4A, or DQ@Asp 10 SAC4A. C ) Representative SA-β-Gal staining images of senescent cells treated with different formulations under normoxic and hypoxic conditions, and quantification of SA-β-Gal-positive area (%) ( n = 4/group). Scale bar: 100 μm. D–F ) Representative Western blot images ( D ) and quantitative analyses ( E , F ) of senescence markers P16 and P21 expression in different treatment groups ( n = 3/group). G ) Representative alkaline phosphatase (ALP, upper panel) and Alizarin Red staining (ARS, lower panel) images demonstrating osteogenic differentiation after indicated treatments under normoxic and hypoxic conditions. H–J ) Representative Western blot images ( H ) and quantitative analyses ( I , J ) showing protein expression levels of osteogenic markers RUNX2 and osteopontin (OPN) ( n = 3/group). (Data are presented as mean ± SD; * P < 0.05, ** P < 0.01, *** P < 0.001; n = 3–4/group)

    Article Snippet: Primary mouse bone marrow mesenchymal stem cells (BMSCs) were purchased from Servicebio (Catalog number STCC6011P).

    Techniques: In Vitro, Staining, Western Blot, Expressing

    Cells at the earlier stage of osteoclast lineage contribute to MSC recruitment A) Schematic diagram of the Transwell assay. MSCs were seeded in the upper well, and osteoclast lineage cells were seeded in the lower well; B) Transwell migration assay of each osteoclast lineage at different time points; C) Analysis of the number of migrated MSCs when cocultured with different osteoclast lineage cells (from day 1 to day 6); Scale bars: 200 μm; D) Schematic diagram of the coculture assay. MSCs were seeded in the upper well, and osteoclast lineage cells were seeded in the lower well; E) Representative images of ALP staining (panoramic and local) of MSCs under osteogenic and adipogenic induction. Scale bars: 200 μm (upper), 50 μm (bottom). F) Quantification of integrated optical density (IOD) for ALP staining for the six groups (n = 3). All data are shown as the mean ± SD. **P ≤ 0.01, *P < 0.05; NS, not significant (P > 0.05).

    Journal: Journal of Orthopaedic Translation

    Article Title: Osteostaticytes: A novel osteoclast subset couples bone resorption and bone formation

    doi: 10.1016/j.jot.2024.06.010

    Figure Lengend Snippet: Cells at the earlier stage of osteoclast lineage contribute to MSC recruitment A) Schematic diagram of the Transwell assay. MSCs were seeded in the upper well, and osteoclast lineage cells were seeded in the lower well; B) Transwell migration assay of each osteoclast lineage at different time points; C) Analysis of the number of migrated MSCs when cocultured with different osteoclast lineage cells (from day 1 to day 6); Scale bars: 200 μm; D) Schematic diagram of the coculture assay. MSCs were seeded in the upper well, and osteoclast lineage cells were seeded in the lower well; E) Representative images of ALP staining (panoramic and local) of MSCs under osteogenic and adipogenic induction. Scale bars: 200 μm (upper), 50 μm (bottom). F) Quantification of integrated optical density (IOD) for ALP staining for the six groups (n = 3). All data are shown as the mean ± SD. **P ≤ 0.01, *P < 0.05; NS, not significant (P > 0.05).

    Article Snippet: Mouse bone marrow mesenchymal stem cells (BMSCs) were obtained from Procell Life Science & Technology Co., Ltd. (Wuhan, China) and grown in DMEM (Gibco, Carlsbad, California, United States).

    Techniques: Transwell Assay, Transwell Migration Assay, Co-culture Assay, Staining

    OSCs are sufficient for the occurrence of bone mesenchymal stem cells during bone remodeling A) Schematic diagram of the tibia fracture model. B) Representative radiographs of the mouse fracture model via X-ray. C) USA300/Eno-Antares2 tracing bacteria showed the infection condition of fracture lesions every 4 days. D) Bar plot of infection conditions among each time point with USA300/Eno-Antares2 tracing bacteria. All data are shown as the mean ± SD, n = 5. **P ≤ 0.01, *P < 0.05; NS, not significant (P > 0.05). E, F and G) Immunofluorescence staining of IDO1 (red) and CD90 (purple) in normal fracture lesions and infected fracture lesions were stained in sections; E for 2 weeks after fracture, F for 3 weeks after fracture, G for 4 weeks after fracture; Scale bars: 50 μm. H and I) The violin graph shows the statistical results of purple fluorescence intensity (CD90-AF647 nm) representing the number of MSCs (H) and red fluorescence intensity (IDO1-AF568 nm) representing the number of OSCs (I). (n = 5) P values are displayed over the data. BF represents the normal fracture group, and OBF represents the infected fracture group.

    Journal: Journal of Orthopaedic Translation

    Article Title: Osteostaticytes: A novel osteoclast subset couples bone resorption and bone formation

    doi: 10.1016/j.jot.2024.06.010

    Figure Lengend Snippet: OSCs are sufficient for the occurrence of bone mesenchymal stem cells during bone remodeling A) Schematic diagram of the tibia fracture model. B) Representative radiographs of the mouse fracture model via X-ray. C) USA300/Eno-Antares2 tracing bacteria showed the infection condition of fracture lesions every 4 days. D) Bar plot of infection conditions among each time point with USA300/Eno-Antares2 tracing bacteria. All data are shown as the mean ± SD, n = 5. **P ≤ 0.01, *P < 0.05; NS, not significant (P > 0.05). E, F and G) Immunofluorescence staining of IDO1 (red) and CD90 (purple) in normal fracture lesions and infected fracture lesions were stained in sections; E for 2 weeks after fracture, F for 3 weeks after fracture, G for 4 weeks after fracture; Scale bars: 50 μm. H and I) The violin graph shows the statistical results of purple fluorescence intensity (CD90-AF647 nm) representing the number of MSCs (H) and red fluorescence intensity (IDO1-AF568 nm) representing the number of OSCs (I). (n = 5) P values are displayed over the data. BF represents the normal fracture group, and OBF represents the infected fracture group.

    Article Snippet: Mouse bone marrow mesenchymal stem cells (BMSCs) were obtained from Procell Life Science & Technology Co., Ltd. (Wuhan, China) and grown in DMEM (Gibco, Carlsbad, California, United States).

    Techniques: Bacteria, Infection, Immunofluorescence, Staining, Fluorescence

    Identification of BMSCs-exosomes. (A) Size distribution of BMSCs-exosomes. (B) transmission electron microscopy image of BMSCs-exosomes. Scale bars: 100 nm. (C) Western blot results of exosome surface proteins

    Journal: BMC Musculoskeletal Disorders

    Article Title: Bone marrow mesenchymal stem cell-derived exosomes promote osteoblast proliferation, migration and inhibit apoptosis by regulating KLF3-AS1/miR-338-3p

    doi: 10.1186/s12891-024-07236-0

    Figure Lengend Snippet: Identification of BMSCs-exosomes. (A) Size distribution of BMSCs-exosomes. (B) transmission electron microscopy image of BMSCs-exosomes. Scale bars: 100 nm. (C) Western blot results of exosome surface proteins

    Article Snippet: Primary mouse bone marrow mesenchymal stem cells (BMSCs) were purchased from ATCC and cultured in a humidity-adjustable incubator containing 5% CO 2 at 37°C.

    Techniques: Transmission Assay, Electron Microscopy, Western Blot

    Effects of BMSCs-exosomes on the function of MC3T3-E1 cells. (A) Screening of BMSCs-Exo concentration. (B) BMSCS-EXO-induced KLF3-AS1 elevation can be transfected with si-KLF3-AS1 inversion. BMSCs-Exo can promote the (C) cell viability and (D) migration of MC3T3-E1 cells and inhibit (E) cell apoptosis. * p < 0.05, ** p < 0.01, *** p < 0.001 vs. control group; ## p < 0.01, ### p < 0.001 vs. BMSCs-Exo group ( n = 5, one-way ANOVA).

    Journal: BMC Musculoskeletal Disorders

    Article Title: Bone marrow mesenchymal stem cell-derived exosomes promote osteoblast proliferation, migration and inhibit apoptosis by regulating KLF3-AS1/miR-338-3p

    doi: 10.1186/s12891-024-07236-0

    Figure Lengend Snippet: Effects of BMSCs-exosomes on the function of MC3T3-E1 cells. (A) Screening of BMSCs-Exo concentration. (B) BMSCS-EXO-induced KLF3-AS1 elevation can be transfected with si-KLF3-AS1 inversion. BMSCs-Exo can promote the (C) cell viability and (D) migration of MC3T3-E1 cells and inhibit (E) cell apoptosis. * p < 0.05, ** p < 0.01, *** p < 0.001 vs. control group; ## p < 0.01, ### p < 0.001 vs. BMSCs-Exo group ( n = 5, one-way ANOVA).

    Article Snippet: Primary mouse bone marrow mesenchymal stem cells (BMSCs) were purchased from ATCC and cultured in a humidity-adjustable incubator containing 5% CO 2 at 37°C.

    Techniques: Concentration Assay, Transfection, Migration, Control

    Effects of miR-338-3p in MC3T3-E1 cells incubated with BMSCs-Exo. (A) Transfection of miR-338-3p inhibitors down-regulated the expression level of miR-338-3p in BMSCs-Exo incubated MC3T3-E1 cells. The decrease of miR-338-3p can promote the (B) viability and (C) migration of BMSCs-Exo incubated MC3T3-E1 cells, and inhibit (D) cell apoptosis. *** p < 0.001 vs. Control group; ## p < 0.01, ### p < 0.001 vs. BMSC-Exos + si-NC group; & p < 0.05, && p < 0.01, &&& p < 0.001 vs. BMSC-Exos + si-KLF3-AS1 + inhibitor-NC group ( n = 5, one-way ANOVA).

    Journal: BMC Musculoskeletal Disorders

    Article Title: Bone marrow mesenchymal stem cell-derived exosomes promote osteoblast proliferation, migration and inhibit apoptosis by regulating KLF3-AS1/miR-338-3p

    doi: 10.1186/s12891-024-07236-0

    Figure Lengend Snippet: Effects of miR-338-3p in MC3T3-E1 cells incubated with BMSCs-Exo. (A) Transfection of miR-338-3p inhibitors down-regulated the expression level of miR-338-3p in BMSCs-Exo incubated MC3T3-E1 cells. The decrease of miR-338-3p can promote the (B) viability and (C) migration of BMSCs-Exo incubated MC3T3-E1 cells, and inhibit (D) cell apoptosis. *** p < 0.001 vs. Control group; ## p < 0.01, ### p < 0.001 vs. BMSC-Exos + si-NC group; & p < 0.05, && p < 0.01, &&& p < 0.001 vs. BMSC-Exos + si-KLF3-AS1 + inhibitor-NC group ( n = 5, one-way ANOVA).

    Article Snippet: Primary mouse bone marrow mesenchymal stem cells (BMSCs) were purchased from ATCC and cultured in a humidity-adjustable incubator containing 5% CO 2 at 37°C.

    Techniques: Incubation, Transfection, Expressing, Migration, Control

    PTEN is a target gene of miR-338-3p. (A) Complementary sites of miR-338-3p and PTEN. (B) luciferase reporter gene assay. *** p < 0.001 vs. Control group ( n = 5, one-way ANOVA). The expression level of PTEN in serum was down-regulated in patients with (C) hand fracture and (D) intraarticular fracture (Healthy individuals = 65, Hand fracture = 35, Intra-articular fracture = 30). *** p < 0.001 vs. Healthy individuals (Independent sample t test). (E) The content of PTEN was increased in BMSCs-Exo incubated cells. *** p < 0.001 vs. control group; ### p < 0.001 vs. BMSCs-Exo + si-NC group; &&& p < 0.001 vs. BMSCs-Exo + si-KLF3-AS1 + inhibitor-NC group ( n = 5, one-way ANOVA).

    Journal: BMC Musculoskeletal Disorders

    Article Title: Bone marrow mesenchymal stem cell-derived exosomes promote osteoblast proliferation, migration and inhibit apoptosis by regulating KLF3-AS1/miR-338-3p

    doi: 10.1186/s12891-024-07236-0

    Figure Lengend Snippet: PTEN is a target gene of miR-338-3p. (A) Complementary sites of miR-338-3p and PTEN. (B) luciferase reporter gene assay. *** p < 0.001 vs. Control group ( n = 5, one-way ANOVA). The expression level of PTEN in serum was down-regulated in patients with (C) hand fracture and (D) intraarticular fracture (Healthy individuals = 65, Hand fracture = 35, Intra-articular fracture = 30). *** p < 0.001 vs. Healthy individuals (Independent sample t test). (E) The content of PTEN was increased in BMSCs-Exo incubated cells. *** p < 0.001 vs. control group; ### p < 0.001 vs. BMSCs-Exo + si-NC group; &&& p < 0.001 vs. BMSCs-Exo + si-KLF3-AS1 + inhibitor-NC group ( n = 5, one-way ANOVA).

    Article Snippet: Primary mouse bone marrow mesenchymal stem cells (BMSCs) were purchased from ATCC and cultured in a humidity-adjustable incubator containing 5% CO 2 at 37°C.

    Techniques: Luciferase, Reporter Gene Assay, Control, Expressing, Incubation